Effect of eight weeks of swimming training and consumption of CBD oil on the expression of NF-B and 5­HT1A genes in heart tissue of rats with myocardial infraction

Background and aims: The use of herbal supplements along with exercise is common among people to treat chronic diseases and metabolic disorders. The present study aimed to investigate the effect of eight weeks of swimming training with CBD oil consumption on the expressions of NF-kB and 5-HT1A genes in the heart tissue of rats with myocardial infraction. Methods: In this experimental study, 20 myocardial infraction rats were divided into four groups of five series: 1) control; 2) training; 3) CBD; and 4) CBD + training. To investigate the effects of myocardial infarction induction on the research variables, five healthy rats were placed in a healthy control group. Induction of myocardial ischemia was performed by subcutaneous injection of isoproterenol. CBD groups received 50mg/kg/day of CBD oil by gavage for eight weeks. The training groups performed swimming training for five days a week. Real time-PCR was designed to measure the levels of cardiac tissue gene expression. Results: Although swimming, CBD consumption, and swimming combined with CBD decreased NF-kB gene expression and increased 5-HT1A gene expression in heart tissue (p<0.05), the combination of swimming training with CBD had a more significant effect on them (p<0.05). Conclusion: Therefore, it seems that swimming training and CBD with the positive regulation of NF-kB and negative regulation of 5-HT1A of inflammatory factors in heart tissue can reduce inflammatory factors and improve cardiovascular health.